Three Brazilian sisters with a combined age of 316 have been named by Guinness World Records as the oldest living trio of siblings in the world.
Researchers are now studying the sisters to find out the secret to longevity.
The DNA Longevo Project, a study led by scientist Mayana Zatz from the University of Sao Paulo, aims to investigate the biological factors behind aging.
The sisters, Zulina de Deus Nunes, 103, Zoraide de Deus Mota, 104, and Levita de Deus Nunes, 109, who live in Rio de Janeiro, were identified through LongeviQuest, a global organization that verifies longevity records and partners with Guinness World Records.
The three sisters credit their longevity to a healthy diet and an active lifestyle.
Zulina recalled a childhood spent swimming and fishing in rivers.
"Everything was fresh. We didn't have a refrigerator," she said.
"Breastfeeding is incredibly important," Zoraide added.
The sisters otherwise led fairly ordinary lives. Levita worked as a craftswoman and later at a television network. Zoraide worked as a nurse and raised five children, while Zulina, a stay-at-home mom, raised six.
Levita looks back on her life without regrets.
She said: "I had a good childhood and adolescence. I can't complain."
Findings from the three sisters' case could help scientists better understand why some people remain physically and cognitively resilient at exceptionally advanced ages.
"When sisters reach that age, there is clearly a strong genetic component," said Ben Meyers, CEO of LongeviQuest. "But because they live near each other, they also have a support network, with family able to help when needed. There is definitely a community aspect as well."
Researchers will compare nonagenarians and centenarians with people who have developed frailty, cognitive decline or chronic diseases, seeking traits linked to longevity.
"Through DNA testing, we look for protective genes, and we know there are several of them," said Zatz, who coordinates the university's Human Genome Research Center. "The more people we have who live past 100, especially families with multiple centenarians, the more accurate our research will be in identifying them."
Scientists believe inherited factors may play a larger role than environmental influences in preserving health and function later in life.
Researchers hope to understand how genetic factors, rather than lifestyle, help protect the heart, muscles, and cognitive function from the ravages of aging.
The study's goal, said researcher Joao Paulo Guilherme, who works with Zatz, "is to reach 500 centenarians so we can draw more definitive conclusions about longevity."
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